The Isolation of Nucleoli from Ungerminated Pea Embryos
نویسندگان
چکیده
A method is described for the isolation of nuclei and nucleoli from ungerminated pea embryos. Electron micrographs of the nucleolar preparation indicate a high degree of purity but only a partial preservation of composition, because of a loss of material from the nucleoli which occurs during the isolation procedure.
منابع مشابه
Some Chemical Properties of Isolated Pea Nucleoli
Isolated nuclei and nucleoli of ungerminated pea embryos have been analyzed chemically for their content of DNA, RNA, zinc, iron, phosphorus, and protein sulfhydryl groups. The values obtained cannot be considered to represent the whole of the living nucleolar body as an undetermined amount of material is extracted from nucleoli in the course of their isolation. Only negligible amounts of DNA h...
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عنوان ژورنال:
- The Journal of Biophysical and Biochemical Cytology
دوره 6 شماره
صفحات -
تاریخ انتشار 1959